human paad cell lines Search Results


human alveolar type ii epithelial carcinoma cell line a549  (ATCC)
99
ATCC human alveolar type ii epithelial carcinoma cell line a549
Human Alveolar Type Ii Epithelial Carcinoma Cell Line A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hepes  (Thermo Fisher)
99
Thermo Fisher hepes
Hepes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cre recombination switches expression  (Addgene inc)
93
Addgene inc cre recombination switches expression
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Cre Recombination Switches Expression, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paav ef1a vcre  (Addgene inc)
91
Addgene inc paav ef1a vcre
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Paav Ef1a Vcre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav1 aav 1 syn jgcamp7s wpre addgene addgene  (Addgene inc)
96
Addgene inc aav1 aav 1 syn jgcamp7s wpre addgene addgene
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Aav1 Aav 1 Syn Jgcamp7s Wpre Addgene Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav8 hsyn dio hm4d gi mcherry addgene  (Addgene inc)
96
Addgene inc aav8 hsyn dio hm4d gi mcherry addgene
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Aav8 Hsyn Dio Hm4d Gi Mcherry Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paav mdlx gfp fishell 1  (Addgene inc)
95
Addgene inc paav mdlx gfp fishell 1
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Paav Mdlx Gfp Fishell 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paav cag icre  (Addgene inc)
93
Addgene inc paav cag icre
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Paav Cag Icre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chrimsonr  (Addgene inc)
93
Addgene inc chrimsonr
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Chrimsonr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp nlrp2 hs00215284 m1  (Thermo Fisher)
86
Thermo Fisher gene exp nlrp2 hs00215284 m1
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Gene Exp Nlrp2 Hs00215284 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human serum type ab  (Danaher Inc)
90
Danaher Inc human serum type ab
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Human Serum Type Ab, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hela cells  (ATCC)
99
ATCC hela cells
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Hela Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a , Tα1-Cre and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated recombination occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre expression, the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.

Journal: bioRxiv

Article Title: Fine-scale excitatory cortical circuits reflect embryonic progenitor pools

doi: 10.1101/363069

Figure Lengend Snippet: a , Tα1-Cre and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated recombination occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre expression, the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.

Article Snippet: Plasmid DNA included: (i) ‘Tα1-Cre’, in which the gene for Cre recombinase is under the control of a portion of the Tα1 promoter ; (ii) ‘CβA-FLEx’ which uses the chicken β-actin promoter to control a flexible excision (FLEx) cassette in which Cre recombination switches expression from TdTomato fluorescent protein to enhanced green fluorescent protein ; (iii) ‘DIO-ChR2-mCherry’ (pAAV-EF1a-doublefloxed-hChR2(H134R)-mCherry-WPRE-HGHpA; Addgene #20297), in which Cre recombination turns on the expression of channelrhodopsin-2 (ChR2) under the control of the human elongation factor-1a promoter ; (iv) DO-ChR2-mCherry (‘Cre-Off’; pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA; Addgene #37082 in which Cre recombination turns off the expression of ChR2 under the control of the human elongation factor-1a promoter ; and (v) DIO-ChR2-EYFP (pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA; Addgene #20298), which is equivalent to DIO-ChR2-mCherry, except that EYFP replaces mCherry.

Techniques: Derivative Assay, Plasmid Preparation, Expressing